Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway     

Liu F  Zhong H  Liang JY  Fu P  Luo ZJ  Zhou L  Gou R  Huang J 《Journal of Zhejiang University. Science. B》2010,11(12):905-911

In this paper,we investigate the effect and the possible mechanism of high glucose levels on the calcification of human aortic smooth muscle cells (HASMCs).HASMCs were divided into four groups: normal glucose group (NG),osmolality control group (OC),high glucose group (HG,HASMCs culture medium containing 30 mmol/L glucose),and high glucose plus recombinant human Noggin protein (bone morphogenetic protein-2 (BMP-2) antagonist) group (HN).The mRNA levels and the protein expressions of BMP-2 and core binding factor alpha-1 (Cbfα-1) were measured by real-time quantitative polymerase chain reaction (PCR) and Western blot.After induced by 10 mmol/L β-glycerol phosphoric acid,cells were harvested for assessments of alkaline phosphatase (ALP) activities at Days 1,2,and 3,and intracellular calcium contents at Days 7 and 14,respectively.High glucose levels increased the mRNA levels and the protein expressions of BMP-2 and Cbfα-1 (P0.05).The expression of Cbfα-1 was partially blocked by Noggin protein (P0.05),while BMP-2 was not (P0.05).After being induced by β-glycerol phosphoric acid,high glucose levels increased the ALP activity [(48.63±1.03) vs.(41.42±2.28) U/mg protein,Day 3;P0.05] and the intracellular calcium content [(2.76±0.09) vs.(1.75±0.07) μmol/mg protein,Day 14;P0.05] in a time-dependent manner when compared with the NG group,while the ALP activity could not be blocked by Noggin protein [(48.63±1.03) vs.(47.37±0.97) U/mg protein,Day 3;P0.05].These results show that high glucose levels can evoke the calcification of HASMCs by inducing osteoblastic trans-differentiation and intracellular calcium deposition via the BMP-2/Cbfα-1 pathway,which can be partially blocked by Noggin protein.  相似文献   

Examining Chi-Square Test Statistics Under Conditions of Large Model Size and Ordinal Data     

Dexin Shi  Christine DiStefano  Heather L. McDaniel  Zhehan Jiang 《Structural equation modeling》2018,25(6):924-945

This study examined the effect of model size on the chi-square test statistics obtained from ordinal factor analysis models. The performance of six robust chi-square test statistics were compared across various conditions, including number of observed variables (p), number of factors, sample size, model (mis)specification, number of categories, and threshold distribution. Results showed that the unweighted least squares (ULS) robust chi-square statistics generally outperform the diagonally weighted least squares (DWLS) robust chi-square statistics. The ULSM estimator performed the best overall. However, when fitting ordinal factor analysis models with a large number of observed variables and small sample size, the ULSM-based chi-square tests may yield empirical variances that are noticeably larger than the theoretical values and inflated Type I error rates. On the other hand, when the number of observed variables is very large, the mean- and variance-corrected chi-square test statistics (e.g., based on ULSMV and WLSMV) could produce empirical variances conspicuously smaller than the theoretical values and Type I error rates lower than the nominal level, and demonstrate lower power rates to reject misspecified models. Recommendations for applied researchers and future empirical studies involving large models are provided.  相似文献   

高等职业技术教育改革的几点思考   总被引：2，自引：0，他引：2  

朱卫斌  陈肇太 《青岛职业技术学院学报》2006,19(1):17-20

社会的需求导致了高职教育的快速发展,同时也存在着许多问题,主要表现在:教育模式的陈旧,课程设置的不合理,教学与实践的脱节,考核方法的单一,教师培训的不足等。如果解决不好这些问题,会制约高职教育的持续发展。  相似文献   

RLC串/并联谐振回路的谐振特性与典型参数分析     

黄进文 《保山师专学报》2006,25(5):78-82

RLC串/并联回路是各种复杂网络的基础,也是各种具有频率特性的电路网络的基本组成部份;全面了解RLC串/并联谐振回路各方面的特性对理解、学习及实践电路尤为重要,同时也是一个难点。  相似文献   

联想:散文“形散神聚”的重要心理因素     

吕相康 《黄石理工学院学报(人文社科版)》2002,(2)

对于散文“形散神聚”的基本特点,现行的写作学大多离开写作主体的心理视角,从章法和文法这些形式层面子以阐释。本文以写作主体的心理活动为内在依据,具体论述基于写作意图所展开的合乎心理规律的丰富联想是构成散文“形散神聚”的心理因素,正是写作主体这一心理因素的功能作用,才使作品形成与读者审美心理同态对应的具有复合之美的丰满、多棱、立体的美学效果。  相似文献   

教师期望效应的影响因素、传递机制及中介物质     

颜红  丁蕙 《大学教育科学》2006,(1):87-90

结合国内外的研究，对期望效应中来自师生双方面的影响因素进行了分析，从期望形成的心理成因，期望传递的范畴、渠道和期望实现的中介物质三方面探讨期望效应的实现过程。  相似文献   

福建省制造业竞争力研究——基于因子分析的实证   总被引：1，自引：3，他引：1  

罗辑  张其春 《福建工程学院学报》2006,4(5):487-491

制造业的竞争力已经成为福建省经济持续增长的重要动力和支撑点。文章通过构建制造业竞争力评估指标体系，采用因子分析对福建省制造业28个行业的竞争力水平进行综合评价，结果显示，福建省制造业相对有竞争力的行业并没有遥遥领先于国内平均水平；福建省重点扶持的产业竞争力总体上还比较弱；福建省的高新技术产业正处于发展之中，尚难在工业增长中充分发挥先导作用；福建主导产业的产业集中度还不是很高，规模经济效应并不是非常明显，产业竞争力尚需大大加强；《海峡西岸经济区建设纲要（试行）》中确定的主导、支柱产业基本上尚未形成优势，竞争力较强的产业基本上是低技术、劳动密集型产业。  相似文献   

光泵磁共振实验评述     

王书运 《实验技术与管理》2007,24(11):35-38

对光磁共振实验的主要内容进行了系统的评述，包括实验中水平方向的磁场、光抽运信号的变化、光泵磁共振信号观察时光抽运信号混杂出现和谐波共振的情况、gF因数的测量方法，以及用光磁共振和光抽运信号测地磁场的方法。  相似文献   

影响大学生就业因素的研究   总被引：2，自引：0，他引：2  

杨金梅  胡冬梅  张楠 《职业技术教育》2006,(25):70-73

问卷调查及访谈结果显示，大学生获取就业信息途径呈现多样化特点；影响大学生就业的各种因素依次为个人因素、用人单位因素、教育因素、社会因素、家庭因素和地理因素。为此，大学生要注重综合素质的提高，高校应重视毕业生质量与就业指导，用人单位要合理用才，家庭要发挥指导作用。  相似文献   

四川环境污染与经济增长的计量分析     

姚寿福 《成都师专学报》2008,(6):75-78

环境与经济的关系问题一直是人们关注的焦点。本文通过建立经济增长与环境污染之间的计量模型,运用计量经济方法分析了四川经济增长与环境污染之间的关系及其冲击效应,证实了环境库兹涅茨曲线在四川并不存在。因此,在环境保护和改善方面,政府应发挥积极的作用,即通过制定产业政策、经济结构调整政策和环境改善政策,加强对环境污染项目或企业的控制和环境污染排放的监督检查,促进经济增长方式的转变,实现经济与环境的协调发展。  相似文献